The determination and analysis of the complete mitochondrial genome of Dario dario (Anabantiformes: Badidae)

Qi Wang; Xinyu Zhao; Chuanchen Ren; Fang Meng; Hongqiang Wang; Yifan Liu; Bingjian Liu; Enshang Yang

doi:10.1080/23802359.2021.1981164

The determination and analysis of the complete mitochondrial genome of Dario dario (Anabantiformes: Badidae)

Mitochondrial DNA B Resour. 2021 Sep 27;6(10):3073-3075. doi: 10.1080/23802359.2021.1981164. eCollection 2021.

Authors

Qi Wang^{1

2}, Xinyu Zhao^{1

2}, Chuanchen Ren^{1

2}, Fang Meng^{1

2}, Hongqiang Wang³, Yifan Liu^{1

2}, Bingjian Liu^{1

2}, Enshang Yang⁴

Affiliations

¹ National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.
² National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.
³ Zhoushan Hospital of Zhejiang Province, Zhoushan, China.
⁴ School of Marine Engineering Equipment, Zhejiang Ocean University, Zhoushan, China.

Abstract

The classification of Badidae family based on morphology has been revised several times, but data on complete mitogenome are scarce, the complete mitochondrial genome of the Badidae fish Dario dario was characterized for the first time in the present study. The whole mitogenome was 16,830 bp in size and consisted of 13 protein-coding genes, 22 tRNAs, two rRNAs genes, a control region and origin of light-strand replication. The proportion of coding sequences with a total length of 11,431 bp was 67.92%, which encoded 3800 amino acids. The genome composition was highly A + T biased (58.12%), and exhibited a negative AT-skew (-0.0045) and GC-skew (-0.2347). All protein-coding genes started with ATG except for GTG in CO1, while stopped with the standard TAN codons or a single T. The control region (D-loop) ranging from 15,658 bp to 16,830 bp was 1173 bp in size. Phylogenetic analysis showed that D. dario was most closely related to Badis badis. The complete mitochondrial genome sequence provided new insight into taxonomic classification, and a more complex picture of species diversity within the Anabantiformes.

Keywords: Anabantiformes; Badidae; Dario dario; mitochondrial genome; phylogenetic relationship.

Grants and funding

This research was supported by Zhejiang Provincial Natural Science Foundation of China [Nos. LY20C190008, LY19H160012], Science and Technology Project of Zhoushan [No. 2020C21016], Medical and Health Science and Technology Project of Zhejiang Province [No. 2019337264], Science and Technology Project of Traditional Chinese Medicine of Zhejiang Province [No. 2018ZA126], Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang [Nos. 20200201, 20200202], and Starting Research Fund from the Zhejiang Ocean University.