Instillation of Particulate Suspensions to the Lungs

Etsushi Kuroda; Yasuo Morimoto; Ken J Ishii

doi:10.21769/BioProtoc.2618

Instillation of Particulate Suspensions to the Lungs

Bio Protoc. 2017 Nov 20;7(22):e2618. doi: 10.21769/BioProtoc.2618.

Authors

Etsushi Kuroda^{1

2}, Yasuo Morimoto³, Ken J Ishii^{1

2}

Affiliations

¹ Laboratory of Adjuvant Innovation, Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
² Laboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
³ Department of Occupational Pneumology, University of Occupational and Environmental Health, Japan, Fukuoka, Japan.

Abstract

Inhaled fine particulates are thought to cause chronic pulmonary inflammation through the deposition of particulates into the lungs. To investigate the effect of fine particulates on the lungs, instillation of suspension of particulates into the lungs is required. This protocol describes direct injection of suspensions of fine particulates into the airway. We also show examples of typical lung immune responses after particulate administration.

Keywords: Intranasal administration; Lung inflammation; Particulates; Pulmonary aspiration.