Scanning Electron Microscope (SEM) Imaging to Determine Inflorescence Initiation and Development in Olive

Amnon Haberman; Einat Zelinger; Alon Samach

doi:10.21769/BioProtoc.2575

Scanning Electron Microscope (SEM) Imaging to Determine Inflorescence Initiation and Development in Olive

Bio Protoc. 2017 Oct 5;7(19):e2575. doi: 10.21769/BioProtoc.2575.

Authors

Amnon Haberman¹, Einat Zelinger², Alon Samach¹

Affiliations

¹ The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
² CSI Center for Scientific Imaging, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

Abstract

Here we present a protocol that describes how to image the structure of the olive axillary bud meristem with a scanning electron microscope (SEM) in order to characterize its identity and developmental stage. Briefly, the specimen is fixed with glutaraldehyde, saturated with ethanol, dried in a critical point dryer (CPD) system, dissected, coated with a conducting material and imaged with a scanning electron microscopy (SEM).

Keywords: Bud; Flowering; Inflorescence; Meristem; Olive; SEM; Scanning Electron Microscopy.