Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus Persisters

Ashelyn E Sidders; Lauren C Radlinski; Sarah E Rowe; Brian P Conlon

doi:10.1007/978-1-0716-1621-5_15

Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus Persisters

Methods Mol Biol. 2021:2357:223-236. doi: 10.1007/978-1-0716-1621-5_15.

Authors

Ashelyn E Sidders¹, Lauren C Radlinski¹, Sarah E Rowe¹, Brian P Conlon^{2

3}

Affiliations

¹ Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
² Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. brian_conlon@med.unc.edu.
³ Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. brian_conlon@med.unc.edu.

PMID: 34590262
DOI: 10.1007/978-1-0716-1621-5_15

Abstract

Aminoglycosides are bactericidal drugs which require a proton motive force (PMF) for uptake into the bacterial cell. Low energy cells, such as persisters, maintain a PMF below the threshold for drug uptake and are tolerant to aminoglycosides. In this chapter, we discuss mechanisms to target the bacterial membrane and stimulate aminoglycoside uptake to kill Staphylococcus aureus persisters.

Keywords: Adjuvants; Aminoglycosides; Persisters; Staphylococcus aureus; Synergy.

MeSH terms

Aminoglycosides / pharmacology
Anti-Bacterial Agents / pharmacology
Humans
Microbial Sensitivity Tests
Staphylococcal Infections* / drug therapy
Staphylococcus aureus*

Substances

Aminoglycosides
Anti-Bacterial Agents