Amplification, sequencing and characterization of pectin methyl esterase inhibitor 51 gene in Tectona grandis L.f

Nuzhat Bano; Shamim Akhtar Ansari; Abeer Hashem; Elsayed Fathi Abd Allah; Mohammad Israil Ansari

doi:10.1016/j.sjbs.2021.07.015

Amplification, sequencing and characterization of pectin methyl esterase inhibitor 51 gene in Tectona grandis L.f

Saudi J Biol Sci. 2021 Oct;28(10):5451-5460. doi: 10.1016/j.sjbs.2021.07.015. Epub 2021 Jul 10.

Authors

Nuzhat Bano¹, Shamim Akhtar Ansari¹, Abeer Hashem^{2

3}, Elsayed Fathi Abd Allah⁴, Mohammad Israil Ansari⁵

Affiliations

¹ Institute of Forest Productivity, PO; Lalgutwa, NH-23, Ranchi 835303, India.
² Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
³ Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, Agriculture Research Center, Giza, Egypt.
⁴ Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
⁵ Department of Botany, University of Lucknow, Lucknow 226 007, India.

Abstract

Tectona grandis L.f. (Teak), a very important source of incomparable timber, withstands a wide range of tropical deciduous conditions. We achieved partial amplification of pectin methylesterase inhibitor 51 (PMEI) gene in teak by E. pilularis cinnamoyl Co-A reductase (CCR) gene specific primer. The amplified teak gene was of 750 bp, 79% identity and 97% query cover with PMEI of Sesamum indicum. The phylogenetic tree clustered the amplified gene with PMEI of database plant species, Erythranthe guttata and Sesamum indicum (87% bootstrap value). On conversion to amino acid sequence, the obtained protein comprised 237 amino acids. However, PMEI region spanned from 24 to 171 amino acids, 15.94 kDa molecular weight, 8.97 pI value and C₆₉₇H₁₁₁₇N₁₉₉O₂₁₁S₉ molecular formula with four conserved cysteine residues as disulfide bridges. 25.9 % protein residues were hydrophilic, 42.7% hydrophobic and 31.2% neutral. Teak 3D PMEI protein structure corresponded well with Arabidopsis thaliana and Actinidia deliciosa PMEIs. The gene maintains integrity of pectin component of middle lamella of primary cell wall and confers tolerance against various kinds of stresses. Teak conferred with overexpression of PMEI may secure a wide adaptability as well as luxuriant timber productivity and quality in adverse/ fluctuating/ scarce climatic and environmental conditions of tropical forests.

Keywords: 3D PMEI protein structure; Bootstrap analysis Nucleotide sequence; Phylogenetic tree; Ramachandran plot; Stress tolerance.