Separation of a new triterpenoid saponin together with six known ones from Clematis tangutica (Maxim.) Korsh and evaluation of their cytotoxic activities

Nat Prod Res. 2023 Feb;37(3):375-382. doi: 10.1080/14786419.2021.1984468. Epub 2021 Sep 29.

Abstract

A new triterpenoid saponin, 3-O-β-D-allopyranosyl (1→3)-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (IV), together with six known ones Hederacholichiside F (I), Tanguticoside B (II), Tauroside St-H1 (III), Hederoside H1 (V), Kalopanaxsaponin G (VI), Hederasaponin B (VII) were separated from Clematis tangutica (Maxim.) Korsh. Their cytotoxic activities were evaluated. Saponins IV (new compound) and I showed selective inhibitory activities against HGC-27 with IC50 values of 20.17 and 66.18 μM. Saponin VII exhibited extensive inhibitory action against HGC-27, Hela and SK-OV-3 with IC50 values of 16.47-71.36 μM. Saponin III showed selective inhibitory activity against SK-OV-3 with the IC50 value of 48.70 μM. All isolated saponins were inactive (IC50 >150 μM) to GES-1.

Keywords: Clematis tangutica (Maxim.) Korsh; cytotoxic activities; high-speed counter-current chromatography; new compound; triterpenoid saponins.

MeSH terms

  • Antineoplastic Agents*
  • Clematis*
  • Humans
  • Molecular Structure
  • Saponins* / pharmacology
  • Triterpenes* / pharmacology

Substances

  • Saponins
  • Triterpenes
  • Antineoplastic Agents