Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B

Alma Jahić Mujkić; Magda Tušek Žnidarič; Selma Berbić; Eva Žerovnik

doi:10.3390/antiox10091471

Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B

Antioxidants (Basel). 2021 Sep 15;10(9):1471. doi: 10.3390/antiox10091471.

Authors

Alma Jahić Mujkić^{1

2}, Magda Tušek Žnidarič³, Selma Berbić², Eva Žerovnik^{1

4}

Affiliations

¹ Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
² Department of Biochemistry, Faculty of Pharmacy, University of Tuzla, Univerzitetska 1, 75000 Tuzla, Bosnia and Herzegovina.
³ Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia.
⁴ Jožef Stefan International Postgraduate School, Jamova 39, 1000 Ljubljana, Slovenia.

Abstract

In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregates upon overexpression, under stressful conditions and some progressive myoclonus epilepsy of tape 1-EPM1-missense mutations). In addition to the chosen polyphenol, this time, we added a proven antioxidant concentration of 0.5 mM vitC into the fibrillation mixture and varied concentrations of resveratrol, quercetin, and curcumin. Synergy with vitC was observed with curcumin and quercetin.

Keywords: ascorbic acid; curcumin; human stefin B; inhibition of amyloid fibril formation; polyphenols; progressive myoclonus epilepsy of type 1—EPM1; protein aggregation; quercetin; resveratrol; synergistic effect of antioxidants; vitamin C.