Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

Elena N Sigida; Ibrahim M Ibrahim; Maxim S Kokoulin; Hussein H Abulreesh; Khaled Elbanna; Svetlana A Konnova; Yulia P Fedonenko

doi:10.3390/md19090508

Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

Mar Drugs. 2021 Sep 7;19(9):508. doi: 10.3390/md19090508.

Authors

Elena N Sigida^{1

2}, Ibrahim M Ibrahim^{3

4}, Maxim S Kokoulin⁵, Hussein H Abulreesh^{6

7}, Khaled Elbanna^{4

6

7}, Svetlana A Konnova^{1

3}, Yulia P Fedonenko^{1

3}

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia.
² N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, 119991 Moscow, Russia.
³ N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, 410012 Saratov, Russia.
⁴ Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
⁵ G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 let Vladivostoku, 690022 Vladivostok, Russia.
⁶ Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
⁷ Research Laboratories Unit, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

Abstract

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol-water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, TOCSY, ROESY, ¹H,¹³C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.

Keywords: 4-O-[1-carboxyethyl]-d-mannose; O-specific polysaccharide; Salinivibrio; halophilic bacterium; lipopolysaccharide; structure.

MeSH terms

Animals
Aquatic Organisms
Egypt
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Mannose / chemistry
Structure-Activity Relationship
Vibrionaceae*

Substances

Lipopolysaccharides
Mannose