RNA electroelution: Comparing two electroeluter models

Amber N Rogers; Maya K Mastronardo; Tsion G Mekonnen; Ana Maria Soto

doi:10.1016/j.ab.2021.114391

RNA electroelution: Comparing two electroeluter models

Anal Biochem. 2021 Nov 1:632:114391. doi: 10.1016/j.ab.2021.114391. Epub 2021 Sep 22.

Authors

Amber N Rogers¹, Maya K Mastronardo¹, Tsion G Mekonnen², Ana Maria Soto³

Affiliations

¹ Molecular Biology, Biochemistry & Bioinformatics Program, Towson University, Towson, MD, 21252, USA.
² Department of Biological Sciences, Towson University, Towson, MD, 21252, USA.
³ Molecular Biology, Biochemistry & Bioinformatics Program, Towson University, Towson, MD, 21252, USA; Department of Chemistry, Towson University, Towson, MD, 21252, USA. Electronic address: asoto@towson.edu.

PMID: 34562467
DOI: 10.1016/j.ab.2021.114391

Abstract

RNA represents a vibrant area of research and many studies use techniques that require large amounts of purified RNA. One common purification method involves slicing a section of a polyacrylamide gel containing the RNA of interest and eluting the RNA out of the gel using electroelution. Various electroeluter models are available but sometimes a given model becomes discontinued, compelling researchers to choose a different model. Here, we have compared two electroeluters with different chamber designs for their ability to recover RNA from gel pieces. Our results show that both electroeluters are effective and recover comparable amounts of purified RNA.

Keywords: Electroelution; Electrophoresis; Gel elution; PAGE; Polyacrylamide; RNA purification.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry*
Bacillus subtilis / chemistry*
Electrophoresis, Polyacrylamide Gel*
Mycobacterium tuberculosis / chemistry*
RNA, Bacterial / chemistry
RNA, Bacterial / isolation & purification*

Substances

Acrylic Resins
RNA, Bacterial
polyacrylamide gels