Physicochemical and biological evaluation of a triazine-methacrylate monomer into a dental resin

Isadora Martini Garcia; Vicente Castelo Branco Leitune; Victória Britz Rücker; Júlia Nunes; Fernanda Visioli; Fabrício Mezzomo Collares

doi:10.1016/j.jdent.2021.103818

Physicochemical and biological evaluation of a triazine-methacrylate monomer into a dental resin

J Dent. 2021 Nov:114:103818. doi: 10.1016/j.jdent.2021.103818. Epub 2021 Sep 21.

Authors

Isadora Martini Garcia¹, Vicente Castelo Branco Leitune², Victória Britz Rücker³, Júlia Nunes⁴, Fernanda Visioli⁴, Fabrício Mezzomo Collares⁵

Affiliations

¹ Department of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul. Ramiro Barcelos Street, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: isadora.garcia@ufrgs.br.
² Department of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul. Ramiro Barcelos Street, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: vicente.leitune@ufrgs.br.
³ Department of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul. Ramiro Barcelos Street, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: victoria.britz@ufrgs.br.
⁴ Oral Pathology Department, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil.
⁵ Department of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul. Ramiro Barcelos Street, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: fabricio.collares@ufrgs.br.

PMID: 34560223
DOI: 10.1016/j.jdent.2021.103818

Abstract

Objectives: This study aimed to (1) formulate blend resins with 2.5 or 5 wt.% of the methacrylate monomer 1,3,5-triacryloylhexahydro-1,3,5-triazine (TAT), and (2) to evaluate the blend resins regarding the physicochemical and biological properties.

Methods: The base resin was formulated mixing 60 wt.% of bisphenol A glycol dimethacrylate and 40 wt.% of triethylene glycol dimethacrylate with photoinitiator/co/initiator system. TAT was added at 2.5 (G_2.5%) or 5 (G_5%) wt.%, and a group without TAT was used as control (G_ctrl). The resins were analyzed for degree of conversion (DC), Knoop hardness (KHN), softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), antibacterial activity against Streptococcus mutans biofilm formation, and cytotoxicity against human keratinocytes.

Results: There was no difference for the DC (p = 0.676). The addition of TAT at 5 wt.% induced higher KHN (p<0.001), higher resistance against softening in solvent (p<0.001), and higher UTS (p = 0.04). There were no statistically significant differences for contact angle with water (p = 0.106), α-bromonaphtalene (p = 0.454), and SFE (p = 0.172). The higher the TAT concentration, the higher the antibacterial activity (p<0.001). G_2.5% showed no cytotoxicity compared to G_ctrl (p>0.05), and G_5% induced lower cell viability (p<0.05).

Conclusions: The addition of 2.5 wt.% of TAT is suitable for conveying antibacterial activity for dental resins without changing the physicochemical properties or impairing the cytotoxic effect.

Clinical relevance: Methacrylate monomers that decrease bacterial viability and copolymerize with the resin matrix are exciting approaches to developing therapeutic materials. TAT showed promising properties to may hamper and prevent carious lesions when incorporated into dental materials. Further evaluations with higher cariogenic challenges will be carried to analyze the formulated materials.

Keywords: Anti-bacterial agents; Dental caries; Pit and fissure sealants; Polymers; Resins; Rynthetic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Composite Resins / toxicity
Humans
Materials Testing
Methacrylates*
Resins, Synthetic / toxicity
Streptococcus mutans
Triazines* / pharmacology

Substances

Anti-Bacterial Agents
Composite Resins
Methacrylates
Resins, Synthetic
Triazines