Draft genome sequence data of Paenibacillus cisolokensis strain LC2-13A and Xylanibacillus composti strain K-13

Ayaka Uke; Chinda Chhe; Sirilak Baramee; Chakrit Tachaapaikoon; Patthra Pason; Rattiya Waeonukul; Khanok Ratanakhanokchai; Akihiko Kosugi

doi:10.1016/j.dib.2021.107361

Draft genome sequence data of Paenibacillus cisolokensis strain LC2-13A and Xylanibacillus composti strain K-13

Data Brief. 2021 Sep 8:38:107361. doi: 10.1016/j.dib.2021.107361. eCollection 2021 Oct.

Authors

Ayaka Uke¹, Chinda Chhe^{1

2}, Sirilak Baramee³, Chakrit Tachaapaikoon³, Patthra Pason³, Rattiya Waeonukul³, Khanok Ratanakhanokchai⁴, Akihiko Kosugi^{1

2}

Affiliations

¹ Japan International Research Center for Agricultural Sciences (JIRCAS), Biological Resources and Post-Harvest Division, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
² Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
³ Pilot Plant Development and Training Institute (PDTI), King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand.
⁴ Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand.

Abstract

To discover more efficient degradation processes of lignocellulosic biomass, it is still important to analyze genomic and enzymatic data from bacteria that have strong xylanolytic ability. Here, we present the draft genome sequences of the xylanolytic bacteria Paenibacillus cisolokensis strain LC2-13A and Xylanibacillus composti strain K-13 that are closest to Paenibacillus sp. strain DA-C8, which has strong xylan degradation ability under anaerobic growth conditions. Whole-genome sequencing on the Ion GeneStudio S5 System yielded 277 contigs with total size 5,305,208 bp and G+C content 52.3 mol% for strain LC2-13A and 115 contigs with total size 4,652,266 bp and G+C content of 56.2 mol% for strain K-13. The LC2-13A genome had 5,744 protein-coding sequences (CDSs), 57 tRNAs, and 4 clustered regularly interspaced short palindromic repeats (CRISPRs), and the K-13 genome had 4,388 CDSs, 1 rRNA gene, 45 tRNAs, and 5 CRISPRs. The CDSs of LC2-13A and K-13 encoded the following carbohydrate-active enzymes: 98 and 67 glycoside hydrolases, 31 and 29 glycosyl transferases, 23 and 17 carbohydrate esterases, and 13 and 37 carbohydrate-binding modules, respectively. The whole-genome sequences of LC2-13A and K-13 have been deposited in DDBJ/ENA/GenBank under accession numbers BOVK00000000 and BOVJ00000000. The versions described in this paper are version 1.

Keywords: Cellulase; GH family; Glycosyl hydrolase; Paenibacillus cisolokensis; Whole-genome sequencing; Xylanase; Xylanibacillus composti.