Evaluation by MALDI-TOF MS and PCA of the diversity of biosurfactants and their producing bacteria, as adaption to weathered oil components

Shaikha Y Alsayegh; Zulfa Al Disi; Mohammad A Al-Ghouti; Nabil Zouari

doi:10.1016/j.btre.2021.e00660

Evaluation by MALDI-TOF MS and PCA of the diversity of biosurfactants and their producing bacteria, as adaption to weathered oil components

Biotechnol Rep (Amst). 2021 Jul 28:31:e00660. doi: 10.1016/j.btre.2021.e00660. eCollection 2021 Sep.

Authors

Shaikha Y Alsayegh¹, Zulfa Al Disi¹, Mohammad A Al-Ghouti¹, Nabil Zouari¹

Affiliation

¹ Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, PO. Box 2713, Doha, Qatar.

Abstract

Indigenous Qatari bacterial strains were isolated from highly weathered oil-contaminated sites, identified, and differentiated based on their protein profiles using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Their diversity was demonstrated by the principal component analysis (PCA) analysis and establishment of a proteodendogram. Both were based on the protein profile of each strain. Interestingly, this approach also showed diversity within the same subspecies. This high diversity is reflected in the emulsification and solubilization activities of their extracellular biosurfactants. The highest emulsification activity (42.1 ± 2.11 AU/mL) was obtained with a strain of Lysinibacillus fusiformis (SA4) after one week of growth in the minimum salt medium in which diesel (5%) is the sole carbon source, while the highest solubilization activity (9.47% ± 0.47%) was produced by the strain Bacillus subtilis (SA6). The functional diversity of the biosurfactants was demonstrated by PCA analysis which allowed their further clustering based on the Fourier-transform infrared spectroscopy (FTIR) analysis. These findings clearly showed that two types of adaptations occur with hydrocarbons degrading bacteria in the weathered-oily soils, one related to the bacterial cell composition maintaining the biosurfactants composition and one to the biosurfactants, which are the primary tool employed by the cell to interact with the weathered oil. This finding would shed light on the potential and strategies of applications for the bioremediation of highly weathered oil-contaminated soils.

Keywords: Biosurfactants; Hydrocarbon-degrading bacteria; MALDI-TOF MS; PCA; Weathered oil.