Disinfection of Escherichia coli in ice by surface dielectric barrier discharge plasma

Yuntao Guo; Peipei Liu; Liyang Zhang; Siqi Peng; Xinxin Wang; Haiyun Luo; Guizhen Wu

doi:10.1063/5.0064020

Disinfection of Escherichia coli in ice by surface dielectric barrier discharge plasma

Appl Phys Lett. 2021 Aug 30;119(9):090601. doi: 10.1063/5.0064020.

Authors

Yuntao Guo¹, Peipei Liu², Liyang Zhang¹, Siqi Peng¹, Xinxin Wang¹, Haiyun Luo¹, Guizhen Wu²

Affiliations

¹ Department of Electrical Engineering, Tsinghua University, Beijing 100084, China.
² NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Abstract

A variety of pathogens can cause people to suffer from serious diseases, and the transmission of COVID-19 through the cold chain has once again attracted people's attention to cold chain disinfection. Unfortunately, there is no mature cold chain disinfection technique yet. In this study, a low-temperature plasma disinfection technique for a cold chain is proposed. The disinfection effect of plasma generated by surface dielectric barrier discharge on Escherichia coli in ice at cryogenic temperature is studied, and the possible disinfection mechanism is discussed. It is found that the O₃ mode and the NO_x mode also exist in the surface dielectric barrier discharge at cryogenic temperature, just as at room temperature. The disinfection effect of both modes is weak in 5 min plasma treatment, but in 60 min post-treatment, the NO_x mode shows a stronger disinfection effect, with 4.45 log reduction. It is speculated that gaseous H₂O₂ and NO_x can be adsorbed on the ice surface in the NO_x mode and then converted into peroxynitrite, which is a powerful bactericidal species. In conclusion, a low-temperature plasma is a promising technique for cold chain disinfection, which is of great significance for ensuring people's health.