We report here on a new generation of optical ion-selective sensors benefiting from cubosomes or hexosomes-nanostructural lipid liquid phase. Cubosome as well as hexosome optodes offer biocompatibility, self-assembly preparation, high stability in solution, and unique, tunable analytical performance. The temperature trigger reversibly changes the lipid nanoparticle internal structure-changing analyte access to the bulk of the probe and ultimately affecting the response pattern. Thus, cubosome or hexosome optodes are highly promising alternatives to conventional polymeric based optical nanoprobes.