Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran; Olivier Van Aken

doi:10.1007/978-1-0716-1653-6_1

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Methods Mol Biol. 2022:2363:1-12. doi: 10.1007/978-1-0716-1653-6_1.

Authors

Huy Cuong Tran¹, Olivier Van Aken²

Affiliations

¹ Department of Biology, Lund University, Lund, Sweden.
² Department of Biology, Lund University, Lund, Sweden. olivier.van_aken@biol.lu.se.

PMID: 34545481
DOI: 10.1007/978-1-0716-1653-6_1

Abstract

The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.

Keywords: Arabidopsis; Density gradients; Mitochondria; Mitochondrial isolation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis*
Centrifugation, Density Gradient
Mitochondria
Plant Leaves / metabolism
Povidone
Silicon Dioxide

Substances

Percoll
Silicon Dioxide
Povidone