Transcription-translation of the Escherichia coli genome within artificial cells

Chem Commun (Camb). 2021 Oct 7;57(80):10367-10370. doi: 10.1039/d1cc04401j.

Abstract

Here we created artificial cells in which information of the genome of living cells is expressed by the elements encoded in the genome. We confirmed that the system works normally within artificial cells, which paves the way for reconstructing living cells from biomolecules.

MeSH terms

  • Artificial Cells / metabolism*
  • Escherichia coli / genetics*
  • Genes, Bacterial / physiology
  • Genome, Bacterial / physiology*
  • Glycolysis / genetics
  • Luciferases / genetics
  • Luciferases / metabolism
  • Protein Biosynthesis / physiology*
  • RNA, Messenger / metabolism
  • Transcription, Genetic / physiology*

Substances

  • RNA, Messenger
  • Luciferases
  • nanoluc