Lipidomic Analysis of Caenorhabditis elegans Embryos

Hung-Chi Yang; Cheng-Yu Hung; Yi-Yun Pan; Szecheng J Lo; Daniel Tsun-Yee Chiu

doi:10.21769/BioProtoc.2554

Lipidomic Analysis of Caenorhabditis elegans Embryos

Bio Protoc. 2017 Sep 20;7(18):e2554. doi: 10.21769/BioProtoc.2554.

Authors

Hung-Chi Yang^{1

2}, Cheng-Yu Hung¹, Yi-Yun Pan³, Szecheng J Lo⁴, Daniel Tsun-Yee Chiu^{1

3

5

6}

Affiliations

¹ Healthy Aging Research Center, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
² Department of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, Hsinchu, Taiwan.
³ Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
⁴ Department of Biomedical Sciences, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
⁵ Department of Pediatric Hematology/Oncology, Linkou Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
⁶ Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Kwei-San, Tao-Yuan, Taiwan.

Abstract

Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is simple and homogeneous. Here, we demonstrate detailed procedures of C. elegans culture, embryo isolation, lipid extraction and metabolomic data analysis.

Keywords: C. elegans; Embryo; LC-MS; Lipid; Untargeted metabolomic.