Rice Lamina Joint Inclination Assay

Hsing-Yi Li; Hsin-Mei Wang; Seonghoe Jang

doi:10.21769/BioProtoc.2409

Rice Lamina Joint Inclination Assay

Bio Protoc. 2017 Jul 20;7(14):e2409. doi: 10.21769/BioProtoc.2409.

Authors

Hsing-Yi Li¹, Hsin-Mei Wang¹, Seonghoe Jang^{1

2}

Affiliations

¹ Biotechnology Center in Southern Taiwan (BCST) of Agricultural Biotechnology Research Center (ABRC), Academia Sinica, Tainan, Taiwan.
² Institute of Tropical Plant Science, National Cheng Kung University, Tainan, Taiwan.

Abstract

Brassinosteroids (BRs) promote rice lamina inclination. Recently, we showed that OsBUL1 knockout mutant rice (osbul1) is defective in brassinosteroid signaling ( Jang et al., 2017 ). To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we applied the protocol presented below. The protocol focuses on: (1) how to prepare rice samples for the assay, and (2) how to treat BL exogenously. Finally, we have added a result showing lamina inclination between WT and osbul1 in BL solutions of various concentrations.

Keywords: Bioassay; Brassinosteroid; Lamina inclination; Lamina joint; Rice.