Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Sivathevy Amirthagunabalasingam; Arturo Papaluca; Taramatti Harihar; Dindial Ramotar

doi:10.21769/BioProtoc.2291

Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Bio Protoc. 2017 May 20;7(10):e2291. doi: 10.21769/BioProtoc.2291.

Authors

Sivathevy Amirthagunabalasingam¹, Arturo Papaluca², Taramatti Harihar¹, Dindial Ramotar¹

Affiliations

¹ Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Canada.
² Lady Davis Institute, McGill University, Montréal, Canada.

Abstract

Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters.

Keywords: Autofluorescent drug; C. elegans; Doxorubicin; Drug uptake; Epifluorescent microscopy; Organic cation uptake transporters; RNAi feeding bacteria.