Dissection and Whole Mount Staining of Retina from Neonatal Mice

Mitsutaka Ogawa; Tetsuya Okajima

doi:10.21769/BioProtoc.3034

Dissection and Whole Mount Staining of Retina from Neonatal Mice

Bio Protoc. 2018 Oct 5;8(19):e3034. doi: 10.21769/BioProtoc.3034.

Authors

Mitsutaka Ogawa¹, Tetsuya Okajima¹

Affiliation

¹ Department of Molecular Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Abstract

Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice ( Sawaguchi et al., 2017 ) by modifying the original protocols ( Powner et al., 2012 ; Tual- Chalot et al., 2013 ). This protocol can also be used for whole mount staining of adult retina.

Keywords: Angiogenesis; Retina; Whole mount stain.