miR-190a-3p Promotes Proliferation and Migration in Glioma Cells via YOD1

Comput Math Methods Med. 2021 Sep 4:2021:3957738. doi: 10.1155/2021/3957738. eCollection 2021.

Abstract

Introduction: To investigate the function of miR-190a-3p on the proliferation and migration of glioma.

Methods: Twenty glioma samples and 6 normal brain tissue samples were collected. Normal human glial cell line HEB and glioma cell lines were used for the experiments. We then used TargetScan to predict the target genes of miR-190a-3p. Dual-luciferase reporter assay was also used to validate.

Results: Combined with dual-luciferase reporter experiment, we finally verified that YOD1 was the aim, and it was low-expressed in glioma. Besides, a series of mechanism experiments then proved that miR-190a-3p negatively regulates YOD1 expression.

Conclusions: Our research was the first to demonstrate the promoting function of miR-190a-3p in the proliferation and migration of glioma and provided new views for the treatment of glioma. miR-190a-3p was expected to be a new target for molecular therapy of glioma.

Publication types

  • Retracted Publication

MeSH terms

  • Brain Neoplasms / genetics*
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Computational Biology
  • Disease Progression
  • Endopeptidases / genetics*
  • Endopeptidases / metabolism
  • Gene Expression Regulation, Neoplastic
  • Glioma / genetics*
  • Glioma / metabolism
  • Glioma / pathology*
  • Humans
  • MicroRNAs / antagonists & inhibitors
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Targeted Therapy
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology
  • Thiolester Hydrolases / genetics*
  • Thiolester Hydrolases / metabolism

Substances

  • MIRN190 microRNA, human
  • MicroRNAs
  • YOD1 protein, human
  • Thiolester Hydrolases
  • Endopeptidases