Retroviral Transduction of NKT Hybridoma Cells

Ke Wang; Rong Jin; Qing Ge

doi:10.1007/978-1-0716-1775-5_3

Retroviral Transduction of NKT Hybridoma Cells

Methods Mol Biol. 2021:2388:27-34. doi: 10.1007/978-1-0716-1775-5_3.

Authors

Ke Wang^{1

2}, Rong Jin¹, Qing Ge^{3

4}

Affiliations

¹ Department of Immunology, School of Basic Medical Sciences, Peking University, NHC Key Laboratory of Medical Immunology (Peking University), Beijing, China.
² Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.
³ Department of Immunology, School of Basic Medical Sciences, Peking University, NHC Key Laboratory of Medical Immunology (Peking University), Beijing, China. qingge@bjmu.edu.cn.
⁴ Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China. qingge@bjmu.edu.cn.

PMID: 34524659
DOI: 10.1007/978-1-0716-1775-5_3

Abstract

Natural killer T (NKT) cells have been shown to bridge innate and adaptive immunity. However, the rare population and hard-to-transfect of primary NKT cells slow down our understanding of cellular and molecular mechanisms of NKT development and function. To overcome these drawbacks, NKT hybridomas, especially DN32.D3 cells, are applied to study NKT cells in vitro and becoming a valuable tool. Here, we describe the method in the genetic manipulation of DN32.D3 cells by retrovirus, including the generation and concentration of retrovirus, retroviral transduction of DN32.D3 cells, and evaluation of transduction efficiency.

Keywords: DN32.D3; NKT hybridomas; Retroviral transduction; Vector; iNKT.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptive Immunity
Animals
Hybridomas
Mice
Mice, Inbred C57BL
Natural Killer T-Cells*
Retroviridae / genetics