Efficient poly(3-hydroxybutyrate-co-lactate) production from corn stover hydrolysate by metabolically engineered Escherichia coli

Ju Wu; Xiangju Wei; Pengye Guo; Aiyong He; Jiaxing Xu; Mingjie Jin; Yanjun Zhang; Hui Wu

doi:10.1016/j.biortech.2021.125873

Efficient poly(3-hydroxybutyrate-co-lactate) production from corn stover hydrolysate by metabolically engineered Escherichia coli

Bioresour Technol. 2021 Dec:341:125873. doi: 10.1016/j.biortech.2021.125873. Epub 2021 Sep 2.

Authors

Ju Wu¹, Xiangju Wei¹, Pengye Guo¹, Aiyong He², Jiaxing Xu², Mingjie Jin³, Yanjun Zhang⁴, Hui Wu⁵

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
² Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China.
³ School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing 210094, China.
⁴ Institute of Plant Genetics and Developmental Biology, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
⁵ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China; Key Laboratory of Bio-based Material Engineering of China National Light Industry Council, 130 Meilong Road, Shanghai 200237, China. Electronic address: hwu@ecust.edu.cn.

PMID: 34523584
DOI: 10.1016/j.biortech.2021.125873

Abstract

Poly(3-hydroxybutyrate-co-lactate)[P(3HB-co-LA)], is a biodegradable and biocompatible bioplastic, and the monomeric composition of the copolymer plays an important role in affecting its mechanical properties. Corn stover hydrolysate (CSH), the waste by-product in agriculture, has been considered as an important carbon source for value-added biochemical production. Therefore, the effect of CSH on P(3HB-co-LA) biosynthesis was investigated in this study. Taking CSH as the carbon source, the lactate (LA) fraction in the copolymer reached 7.1 mol% by the engineered stain. The results of shake flask fermentation demonstrated that reducing the activity of electron transport system resulted in a higher LA fraction. Furthermore, we replaced the promoter of the key gene pct_th with ldhA gene promoter, so that the expression of pct_th gene could be dynamically modulated as well as the lactic acid content changed. This study suggests that CSH is a promising carbon source for the production of biodegradable P(3HB-co-LA).

Keywords: Corn stover hydrolysate; E. coli; Lactate fraction; Poly(3-hydroxybutyrate-co-lactate); Promoter of ldhA.

MeSH terms

3-Hydroxybutyric Acid
Escherichia coli* / genetics
Lactic Acid*
Polyesters
Zea mays

Substances

Polyesters
Lactic Acid
3-Hydroxybutyric Acid