Nitrifier denitrification dominates nitrous oxide production in composting and can be inhibited by a bioelectrochemical nitrification inhibitor

Abstract

Targeted options to reduce nitrous oxide (N₂O) emission from composting is scarce due to challenges in disentangling the complex N₂O production pathways. Here, combined approaches of nitrogen form analysis, isotopocule mapping, quantitative PCR, and Illumina MiSeq sequencing were used to differentiate N₂O production pathways and decipher the underlying biochemical mechanisms. Results suggested that most N₂O was produced at the latter stage through nitrifier denitrification. The bioelectrochemical assistance through applying an electric potential reduced N₂O emissions by 28.5-75.5%, and the underlying mitigation mechanism was ammonia oxidation repression, as evidenced by the observed reduction in the proportion of the amoA containing family Nitrosomonadaceae from 99% to 83% at the lower voltage and to a negligible level at the higher voltage assessed, which was attributed to their depressed competitiveness for oxygen with heterotrophs. The findings provide evidence that the bioelectrochemical assistance could function as a nitrification inhibitor to minimize compost derived N₂O emissions.

Keywords: Bioelectrochemical assistance; Composting; N(2)O mitigation; Nitrification inhibitor; Nitrifier denitrification.