Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

Abstract

The effect of drought on the interplay of processes controlling carbon partitioning into plant primary and secondary metabolisms, such as respiratory CO₂ release and volatile organic compound (VOC) biosynthesis, is not fully understood. To elucidate the effect of drought on the fate of cellular C sources into VOCs vs CO₂ , we conducted tracer experiments with ¹³ CO₂ and position-specific ¹³ C-labelled pyruvate, a key metabolite between primary and secondary metabolisms, in Scots pine seedlings. We determined the stable carbon isotope composition of leaf exchanged CO₂ and VOC. Drought reduced the emission of the sesquiterpenes α-farnesene and β-farnesene but did not affect ¹³ C-incorporation from ¹³ C-pyruvate. The labelling patterns suggest that farnesene biosynthesis partially depends on isopentenyl diphosphate crosstalk between chloroplasts and cytosol, and that drought inhibits this process. Contrary to sesquiterpenes, drought did not affect emission of isoprene, monoterpenes and some oxygenated compounds. During the day, pyruvate was used in the TCA cycle to a minor degree but was mainly consumed in pathways of secondary metabolism. Drought partly inhibited such pathways, while allocation into the TCA cycle increased. Drought caused a re-direction of pyruvate consuming pathways, which contributed to maintenance of isoprene and monoterpene production despite strongly inhibited photosynthesis. This underlines the importance of these volatiles for stress tolerance.

Keywords: Pinus sylvestris; carbon partitioning; crosstalk; drought; position-specific isotope labelling; terpenes; volatile organic compounds (VOCs).