The utilization of microorganisms like bacteria in the biological synthesis of silver nanoparticles (AgNPs) has attracted widespread attention due to their ability to synthesize different shape sizes, states, and morphology nanoparticles. In the current study, the green synthesis of AgNPs by Nocardiopsis sp. 16S ribosomal RNA analysis was used to characterize the Nocardiopsis sp. The synthesized AgNPs were characterized through multi-instrument platforms such as transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, scanning electron microscope (SEM), and X-ray diffraction analysis (XRD). The antimicrobial activity of the synthesized AgNPs was determined by the agar plate diffusion method. The UV-Vis absorbance analysis of the synthesized AgNPs has a significant absorbance at 384 nm, confirming the AgNPs' surface plasmon resonance. The SEM and TEM characterizations indicate that the particle size ranges from 2 to 10 nm and is spherical. Additionally, the FTIR spectra revealed bands from 476 to 3819cm-1 , respectively. The XRD planes study pronounced strong bands ranging are between 111 and 311 corresponding to cubic face-center of the silver. Also, the antimicrobial activity of AgNPs indicated the biogenic AgNPs could control the growth of the clinical isolates. The AgNPs produced by Nocardiopsis sp. supernatant could be used in different nanomedicinal applications.
Keywords: Nocardiopsis sp.; antimicrobial activity; biosynthesis; silver nanoparticles.
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