A novel selective detection method for sulfide in food systems based on the GMP-Cu nanozyme with laccase activity

Hui Huang; Meini Li; Mingwei Hao; Liangli Lucy Yu; Yongxin Li

doi:10.1016/j.talanta.2021.122775

A novel selective detection method for sulfide in food systems based on the GMP-Cu nanozyme with laccase activity

Talanta. 2021 Dec 1:235:122775. doi: 10.1016/j.talanta.2021.122775. Epub 2021 Aug 2.

Authors

Hui Huang¹, Meini Li², Mingwei Hao², Liangli Lucy Yu³, Yongxin Li⁴

Affiliations

¹ College of Food Science and Engineering, Jilin University, Changchun, 130025, China; Department of Nutrition and Food Science, University of Maryland, College Park, MD, 20742, USA.
² College of Food Science and Engineering, Jilin University, Changchun, 130025, China.
³ Department of Nutrition and Food Science, University of Maryland, College Park, MD, 20742, USA.
⁴ Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun, 130021, China. Electronic address: liyongxin@jlu.edu.cn.

PMID: 34517635
DOI: 10.1016/j.talanta.2021.122775

Abstract

A selective and sensitive colorimetric strategy for sulfide analysis was developed using GMP-Cu nanozymes with a laccase-like activity. This research discovered for the first time that sulfide could significantly enhance the catalytic activity of the GMP-Cu nanozymes by about 3.5 folds. The enhanced laccase activities duo to two reasons. First, Cu²⁺ in GMP-Cu nanozymes was reduced to Cu⁺. The other reason was the formation of Cu-S bond which was beneficial to accelerate the electron transfer rate to improve catalytic activity. Therefore, this method showed an excellent selectivity for sulfide. And it had a linear relationship in the sulfide concentration range of 0-220 μmol/L with a detection limit of 0.67 μmol/L. Furthermore, the proposed method was successfully applied to examine sulfide in the food systems. This new method may be used in sulfide detection to improve food quality and safety.

Keywords: Food system; Laccase; Nanotechnology; Nanozymes; Sulfide detection.

MeSH terms

Colorimetry
Laccase*
Sulfides*

Substances

Sulfides
Laccase