DNase I-assisted 2'-O-methyl molecular beacon for amplified detection of tumor exosomal microRNA-21

Haiyan Zheng; Qingyuan Lin; Jinchao Zhu; Yamin Rao; Liang Cui; Yongyang Bao; Tianhai Ji

doi:10.1016/j.talanta.2021.122727

DNase I-assisted 2'-O-methyl molecular beacon for amplified detection of tumor exosomal microRNA-21

Talanta. 2021 Dec 1:235:122727. doi: 10.1016/j.talanta.2021.122727. Epub 2021 Jul 21.

Authors

Haiyan Zheng¹, Qingyuan Lin¹, Jinchao Zhu¹, Yamin Rao¹, Liang Cui², Yongyang Bao³, Tianhai Ji⁴

Affiliations

¹ Department of Pathology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 20025, China.
² Department of Chemistry, Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, 310018, China. Electronic address: cuiliang@hnu.edu.cn.
³ Department of Pathology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 20025, China. Electronic address: baoyy1294@sh9hospital.org.cn.
⁴ Department of Pathology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 20025, China. Electronic address: skysea_ji@sina.com.

PMID: 34517595
DOI: 10.1016/j.talanta.2021.122727

Abstract

An end-modified 2'-O-methyl molecular beacon (eMB) with unique nuclease resistance was designed and prepared. The eMB can resist the enzymatic digestion by DNase I, which would otherwise occur upon the hybridization of the eMB with a complementary sequence. As a result, the coupling use of eMBs and DNase I allows highly sensitive detection of miRNA with a limit of detection (LOD) of 2.5 pM. The analytical strategy was further used for detection of tumor exosomal microRNA-21, and down to 0.86 μg mL^-1 A375 exosomes were detected. Overall, the present method can effectively quantify tumor-derived exosomes for cancer diagnosis.

Keywords: 2′-O-methyl molecular beacon; DNase I; Exosome; Signal amplification; microRNA.

MeSH terms

Biosensing Techniques*
Deoxyribonuclease I
Exosomes* / genetics
Humans
Limit of Detection
MicroRNAs* / genetics
Neoplasms* / diagnosis
Neoplasms* / genetics

Substances

MIRN21 microRNA, human
MicroRNAs
Deoxyribonuclease I