Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes

Lei Yang; Binglin Xing; Fen Li; Li Kui Wang; Linlin Yuan; Amosi Leonard Mbuji; Zhengqiang Peng; Farag Malhat; Shaoying Wu

doi:10.7717/peerj.12069

Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes

PeerJ. 2021 Aug 24:9:e12069. doi: 10.7717/peerj.12069. eCollection 2021.

Authors

Lei Yang¹, Binglin Xing¹, Fen Li¹, Li Kui Wang¹, Linlin Yuan¹, Amosi Leonard Mbuji^{1

2}, Zhengqiang Peng³, Farag Malhat⁴, Shaoying Wu¹

Affiliations

¹ Hainan University, Haikou, Hainan, China.
² Department of Resources Utilization and Plant Protection, College of Resources and Environmental Science, China Agricultural University, Beijing, Beijing, China.
³ The Ministry of Agriculture and Rural Affairs Key Laboratory of Integrated Pest Management of Tropical Crops, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
⁴ Pesticide Residues and Environmental Pollution Department, Agricultural Research Center, Dokki, Giza, Egypt.

Abstract

Background: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce.

Methods: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes.

Results: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.

Keywords: Detoxification gene; Full-length transcriptome; Resistance; Spodoptera frugiperda.

Grants and funding

This work was supported by the Hainan Major Science and Technology Project (No. ZDKJ202002), the Hainan Provincial Natural Science Foundation of China (No. 320RC473, No. 320RC489), Monitoring and control of major invasive organisms in Hainan Province (No. 1630042020020), Hainan University (No. KYQD (ZR) 1963, No. KYQD (ZR) 1951, No. RZ2100003220). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.