Uncovering viral RNA-host cell interactions on a proteome-wide scale

Louisa Iselin; Natasha Palmalux; Wael Kamel; Peter Simmonds; Shabaz Mohammed; Alfredo Castello

doi:10.1016/j.tibs.2021.08.002

Uncovering viral RNA-host cell interactions on a proteome-wide scale

Trends Biochem Sci. 2022 Jan;47(1):23-38. doi: 10.1016/j.tibs.2021.08.002. Epub 2021 Sep 9.

Authors

Louisa Iselin¹, Natasha Palmalux², Wael Kamel³, Peter Simmonds⁴, Shabaz Mohammed⁵, Alfredo Castello⁶

Affiliations

¹ Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, OX1 3SY, UK; Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK.
² MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, (UK).
³ Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK; MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, (UK).
⁴ Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, OX1 3SY, UK.
⁵ Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK; Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK; The Rosalind Franklin Institute, Oxfordshire, OX11 0FA, UK.
⁶ Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK; MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, (UK). Electronic address: alfredo.castello@glasgow.ac.uk.

Abstract

RNA viruses interact with a wide range of cellular RNA-binding proteins (RBPs) during their life cycle. The prevalence of these host-virus interactions has been highlighted by new methods that elucidate the composition of viral ribonucleoproteins (vRNPs). Applied to 11 viruses so far, these approaches have revealed hundreds of cellular RBPs that interact with viral (v)RNA in infected cells. However, consistency across methods is limited, raising questions about methodological considerations when designing and interpreting these studies. Here, we discuss these caveats and, through comparing available vRNA interactomes, describe RBPs that are consistently identified as vRNP components and outline their potential roles in infection. In summary, these novel approaches have uncovered a new universe of host-virus interactions holding great therapeutic potential.

Keywords: RNA; RNA-binding proteins; proteomics; viral ribonucleoprotein; virus; virus–host interactions.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell Communication
Host Microbial Interactions
Host-Pathogen Interactions
Proteome* / metabolism
RNA, Viral* / genetics
Ribonucleoproteins / metabolism

Substances

Proteome
RNA, Viral
Ribonucleoproteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding