Development of a new dual electrochemical immunosensor for a rapid and sensitive detection of enrofloxacin in meat samples

Abstract

A novel dual electrochemical immunosensor was fabricated for the rapid and sensitive detection of enrofloxacin (EF) antibiotic in meat. Anti-quinolone antibody was immobilized onto screen-printed dual carbon electrodes via carbodiimide coupling. A new electrochemical probe was synthesized by conjugating difloxacin and aminoferrocene, whose oxidation was measured at + 0.2 V vs. Ag/AgCl by differential pulse voltammetry. The detection principle was based on the competitive binding of this conjugate and free EF on immobilized antibodies. The proposed immunosensor allowed detection of EF at concentrations ranging from 0.005 µg.mL^-1 to 0.01 µg.mL^-1 with a detection limit of 0.003 µg.mL^-1. The immunosensor was stable for at least 1 month at 4 °C and displayed a good specificity for other fluoroquinolones. The new dual electrode design offered an improved accuracy as one electrode was used as negative control. The efficiency of the sensor and the adequacy of the extraction process were finally validated by detecting EF in different meat samples.

Keywords: Competitive assay; Enrofloxacin (EF); Fluoroquinolones (FQs); Immunosensor; Meat analysis; Screen-printed dual carbon electrodes (SPdCEs).