Nitrogen form-mediated ethylene signal regulates root-to-shoot K+ translocation via NRT1.5

Abstract

Nitrogen-potassium synergistic and antagonistic interactions are the typical case of nutrient interactions. However, the underlying mechanism for the integration of the external N form into K⁺ homeostasis remains unclear. Here, we show that opposite effects of NO₃^- and NH₄⁺ on root-shoot K⁺ translocation were due to differential regulation of an ethylene signalling pathway targeting the NRT1.5 transporter. NH₄⁺ upregulated the transcriptional activity of EIN3, but repressed the expression of NRT1.5. However, the addition of NO₃^- strongly suppressed the activity of EIN3, whereas its addition upregulated the expression of AtNRT1.5 and shoot K⁺ concentration. The 35S:EIN3/ein3eil1 plants, nrt1.5 mutants and nrt1.5/skor double mutants displayed a low K⁺ chlorosis phenotype, especially under NH₄⁺ conditions with low K⁺ supply. Ion content analyses indicate that root-to-shoot K⁺ translocation was significantly reduced in these mutants. A Y1H assay, an EMSA and a transient expression assay confirmed that AtEIN3 protein could directly bind to the promoter of NRT1.5 to repress its expression. Furthermore, grafted plants with the roots of 35S:EIN3 and ein3eil1/nrt1.5 mutants displayed marked leaf chlorosis with a low K⁺ concentration. Collectively, our findings reveal that the interaction between N form and K⁺ was achieved by modulating root-derived ethylene signals to regulate root-to-shoot K⁺ translocation via NRT1.5.

Keywords: Arabidopsis; N form; leaf senescence; nitrate transport; root-to-shoot K+ translocation.