Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2

Daniele Di Carlo; Laura Mazzuti; Ilaria Sciandra; Giuliana Guerrizio; Giuseppe Oliveto; Rodolfo J Riveros Cabral; Maria Antonella Zingaropoli; Ombretta Turriziani

doi:10.1016/j.jviromet.2021.114276

Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2

J Virol Methods. 2021 Dec:298:114276. doi: 10.1016/j.jviromet.2021.114276. Epub 2021 Sep 6.

Authors

Daniele Di Carlo¹, Laura Mazzuti¹, Ilaria Sciandra¹, Giuliana Guerrizio¹, Giuseppe Oliveto¹, Rodolfo J Riveros Cabral¹, Maria Antonella Zingaropoli², Ombretta Turriziani³

Affiliations

¹ Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
² Department of Public Health and Infectious Diseases, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
³ Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy. Electronic address: ombretta.turriziani@uniroma1.it.

Abstract

The aim of the study was to evaluate the clinical performance of FTD SARS-CoV-2 compared to the RealStar RT-PCR kit 1.0. The analysis of 100 nasopharyngeal swabs showed an overall agreement of 88 %. The positive percentage agreement was 85.6 % and the negative percentage agreement was 91 %. In conclusion we observed a substantial agreement among the two methods, with discrepancies mainly observed in specimens with relatively low amount of viral RNA.

Keywords: Covid-19; Ct values; FTD SARS-CoV-2; RT-PCR; RealStar RT-PCR; SARS-CoV-2.

MeSH terms

COVID-19*
Frontotemporal Dementia*
Humans
Nasopharynx
RNA, Viral / genetics
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Sensitivity and Specificity

Substances

RNA, Viral