Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

Thais P Martins; Tadeu A Souza; Patricia S da Silva; Erich Y T Nakasu; Fernando L Melo; Alice K Inoue-Nagata; Tatsuya Nagata

doi:10.1007/s00705-021-05220-w

Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

Arch Virol. 2021 Nov;166(11):3217-3220. doi: 10.1007/s00705-021-05220-w. Epub 2021 Sep 8.

Authors

Thais P Martins^{1

2}, Tadeu A Souza², Patricia S da Silva², Erich Y T Nakasu², Fernando L Melo¹, Alice K Inoue-Nagata³, Tatsuya Nagata⁴

Affiliations

¹ Department of Molecular Biology, University of Brasilia, Brasilia, DF, Brazil.
² Laboratory of Virology, Embrapa Vegetables, Brasilia, DF, Brazil.
³ Laboratory of Virology, Embrapa Vegetables, Brasilia, DF, Brazil. alice.nagata@embrapa.br.
⁴ Department of Molecular Biology, University of Brasilia, Brasilia, DF, Brazil. tatsuya@unb.br.

PMID: 34498121
DOI: 10.1007/s00705-021-05220-w

Abstract

During a survey in a tomato field in Luziânia (Goiás State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus, named "tomato mottle leaf distortion virus".

MeSH terms

Begomovirus / genetics*
Begomovirus / isolation & purification
Brazil
Genome, Viral
Nanopore Sequencing / methods*
Phylogeny*
Plant Diseases / virology*
Solanum lycopersicum / virology*

Abstract

MeSH terms

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