The present study was conducted to compare the efficiency of different microbial mixed-cultures consists of fifteen oil-degrading microorganisms with different combinations. The investigation was targeted toward the removal of 500 mg/l pyrene and 1% w/v tetracosane, as single compounds or mixture. Sequential Fungal-Bacterial Mixed-Culture (SMC) in which bacteria added one week after fungi, recorded 60.76% and 73.48% degradation for pyrene and tetracosane; about 10% more than Traditional Fungal-Bacterial Mixed-Culture (TMC). Co-degradation of pollutants resulted in 24.65% more pyrene degradation and 6.41% less tetracosane degradation. The non-specified external enzymes of fungi are responsible for initial attacks on hydrocarbons. Delayed addition of bacteria and co-contamination would result in higher growth of fungi which increases pyrene degradation. The addition of Rhamnolipid potently increased the extent of pyrene and tetracosane degradation by approximately 16% and 23% and showed twice better performance than Tween-80 in 20 times less concentration. The results indicated the importance of having sufficient knowledge on the characteristics of the contaminated site and its contaminants as well as oil-degrading species. Gaining this knowledge and using it properly, such as the later addition of bacteria (new method of mixed-cultures inoculation) to the contaminated culture, can serve as a promising approach.
Keywords: Bioremediation; High molecular weight hydrocarbons (HMW); Long-chain aliphatics; Petroleum pollution; Polycyclic aromatic hydrocarbons (PAH).
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