Lipidomic metabolism associated with acetic acid priming-induced salt tolerance in Carex rigescens

Abstract

Acetic acid priming may mitigate salt stress to plants by modulating lipid metabolism. Carex rigescens is a stress-tolerant turfgrass species with a widespread distribution in north China. The objective of this study was to figure out whether modification of lipid profiles, including the contents, compositions and saturation levels of leaf lipids, may contribute to acetic acid modulated salt tolerance in C. rigescens. Plants of C. rigescens were primed with or without acetic acid (30 mM) and subsequently exposed to salt stress (300 mM NaCl) for 15 days. Salt stress affected the physiological performance of C. rigescens, while acetic acid-primed plants showed significantly lower malondialdehyde content, proline content, and electrolyte leakage than non-primed plants under salt stress. Acetic acid priming enhanced the contents of phospholipids and glycolipids involved in membrane stabilization and stress signaling (phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyl diacylglycerol, monogalactosyl diacylglycerol, and sulfoquinovosyldiacylglycerol), reduced the content of toxic lipid intermediates (free fatty acids) during subsequent exposure to salt stress. Furthermore, expression levels of genes involved in lipid metabolism such as CK and PLDα changed due to acetic acid priming. These results demonstrated that acetic acid priming could enhance salt tolerance of C. rigescens by regulating lipid metabolism. The lipids could be used as biomarkers to select for salt-tolerant grass germplasm.

Keywords: Chemical priming; Glycolipids; Phospholipids; Salt stress; Turfgrass.