Functional Heterogeneity of Reelin in the Oral Squamous Cell Carcinoma Microenvironment

Xinwen Zhang; Yong Fu; Zhuang Ding; Nisha Zhu; Mengxiang Zhao; Yuxian Song; Xiaofeng Huang; Sheng Chen; Yan Yang; Caihong Zhang; Qingang Hu; Yanhong Ni; Liang Ding

doi:10.3389/fonc.2021.692390

Functional Heterogeneity of Reelin in the Oral Squamous Cell Carcinoma Microenvironment

Front Oncol. 2021 Aug 17:11:692390. doi: 10.3389/fonc.2021.692390. eCollection 2021.

Authors

Affiliations

¹ Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
² Department of Oral Pathology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
³ Research Institute of Superconductor Electronics, School of Electronic Science and Engineering, Nanjing University, Nanjing, China.

Abstract

Background: Reelin, an extracellular glycoprotein, is expressed on neuronal cells and participates in neuronal migration during brain development. Recently, Reelin also has a vital role in carcinogenesis. However, its role in oral squamous cell carcinoma (OSCC) remains to be explored. The purpose of this study was to explore the roles of Reelin in OSCC.

Methods: The expression of Reelin in cancer-associated fibroblasts (Reelin^CAF) and tumor cells (Reelin^TC) was analyzed by the Gene Expression Omnibus (GEO) database. Immunohistochemistry (IHC) was used to detect the spatial pattern of Reelin in 75 OSCCs. The diagnostic and prognostic values of Reelin were evaluated and also verified by The Cancer Genome Atlas (TCGA) database. Primary CAFs from 13 OSCC patients were isolated to confirm Reelin expression. Thirty-nine OSCC peripheral blood samples were used to analyze the change of immunocytes based on Reelin levels by flow cytometry. The relationship between Reelin and tumor immune microenvironment in head and neck squamous cell carcinoma (HNSCC) tissues was determined by TISIDB and the Tumor Immune Estimation Resource (TIMER) database.

Results: In breast cancer, pancreatic cancer and rectal cancer, Reelin in CAFs was significantly upregulated compared with Reelin in TCs. The IHC results in OSCC also showed that Reelin levels were higher in CAFs. Upregulated Reelin^TC was related to a decreased pN stage and distant metastasis. Strikingly, patients with enhanced Reelin^CAF had a high risk of lymph node metastasis, poor worst pattern of invasion (WPOI), and distant metastasis, but showed comparable Ki-67 level in all OSCC patients, resulting in shorter overall survival (OS) and disease-specific survival (DSS). Unexpectedly, Reelin in tumor-infiltrating lymphocytes (Reelin^TIL) was correlated with postoperative relapse. Patients with high Reelin^TIL, but not Reelin^TC and Reelin^CAF, had poor cytotoxicity of CD8⁺ T cells and higher ratio of CD4/CD8 in peripheral blood. However, Reelin was positively associated with tissue-resident B cells and NK cells in the tumor microenvironment.

Conclusion: Reelin has a versatile function in distinct cell types during the development of OSCC via governing tumor cell and stroma microenvironment.

Keywords: Reelin; cancer-associated fibroblasts; lymphocyte subsets; oral squamous cell carcinoma; prognosis.