Enhanced bio-reductive degradation of fluoroglucocorticoids in the groundwater fluctuation zone by external electron donors: Performance, microbial community, and functional genes

Yayun Xiang; Eldon R Rene; Weifang Ma

doi:10.1016/j.jhazmat.2021.127015

Enhanced bio-reductive degradation of fluoroglucocorticoids in the groundwater fluctuation zone by external electron donors: Performance, microbial community, and functional genes

J Hazard Mater. 2022 Feb 5;423(Pt A):127015. doi: 10.1016/j.jhazmat.2021.127015. Epub 2021 Aug 21.

Authors

Yayun Xiang¹, Eldon R Rene², Weifang Ma³

Affiliations

¹ College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.
² Department of Water Supply, Sanitation and Environmental Engineering, IHE Delft Institute for Water Education, Westvest 7, 2611AX Delft, The Netherlands.
³ College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address: mpeggy@163.com.

PMID: 34482082
DOI: 10.1016/j.jhazmat.2021.127015

Abstract

This study evaluated the effectiveness of external electron donors on the bio-reductive degradation enhancement of fluoroglucocorticoids (FGCs) in the groundwater fluctuation zone during the wet season when reverse upward fluctuation of the groundwater table occurs and the dry season after the groundwater table declines. The results showed that the external electron donors, provided by the addition of nano zero-valent iron-modified biochar (nZVI@BC), inhibited the migration and enhanced the reductive defluorination of triamcinolone acetonide (TA), a representative FGC. The accumulation rate constant with temporal fluctuation depth and the attenuation rate constant with vertical fluctuation depth were -2.55 × 10^-3 and 4.20 × 10^-2, respectively, in the groundwater of the natural groundwater fluctuation zone (N-FZ). In contrast, the accumulation and attenuation rate constants were, respectively, 35.6% and 2.64 times higher in the groundwater fluctuation zone amended with nZVI@BC (nZVI@BC-FZ) as compared with those observed in the N-FZ. Furthermore, the decay rate constant of the TA residue in the dry season was 0.843 × 10^-2 μg/d in N-FZ and was 2.19 times higher in nZVI@BC-FZ. This enhancement effect, caused by the addition of external electrons, was positively correlated with the evolution of the microbial community and the expression of functional genes. The microbes evolved into functional genera with reductive dehalogenation (Xylophilus and Hydrogenophaga) and iron-oxidizing (Lysobacter, Pseudoxanthomonas, and Sphingomonas) abilities in the nZVI@BC-FZ system, which increased dehalogenation and iron oxide genes by a 4-5 order of magnitude. The utilization proportion of external electrons for TA metabolism was 50.04%, of which 30.82%, 10.26%, and 8.96% were utilized for defluorination, hydrogenation, and ring-opening, respectively. This study provides an effective method to reduce pollutant diffusion and enhance the bio-reductive degradation caused by groundwater table fluctuation.

Keywords: Electron donor; Fluoroglucocorticoid; Functional genes; Groundwater fluctuation zone; Reductive defluorination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrons
Groundwater*
Iron
Microbiota*
Oxidation-Reduction
Water Pollutants, Chemical*

Substances

Water Pollutants, Chemical
Iron