Development of alginate dialdehyde-gelatin based bioink with methylcellulose for improving printability

Abstract

This study used methylcellulose (MC) to improve the printability of the alginate dialdehyde-gelatin (ADA-GEL) based bioink. The printability as well as the capability to maintain shape fidelity of ADA-GEL could be enhanced by the addition of 9% (w/v) MC. Moreover, the properties of the ink crosslinked with Ca²⁺ and Ba²⁺ were investigated. The samples crosslinked with Ba²⁺ were more stable and stiffer than the Ca²⁺ crosslinked samples. However, both Ca²⁺ and Ba²⁺ crosslinked samples exhibited a similar trend of MC release during incubation under cell culture conditions. The toxicity test indicated that both samples (crosslinked with Ca²⁺ and Ba²⁺) exhibited no toxic potential. The fabrication of cell-laden constructs using the developed bioinks was evaluated. The viability of ST2 cells in Ba²⁺ crosslinked samples increased while for Ca²⁺ crosslinked samples, a decreased viability was observed over the incubation time. After 21 days, cell spreading in the hydrogels crosslinked with Ba²⁺ occurred. However, a certain degree of cell damage was observed after incorporating the cells in the high viscosity bioink.

Keywords: Alginate; Alginate dialdehyde; Biofabrication; Bioinks; Methylcellulose.