In-depth examination of PrPSc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)

Transbound Emerg Dis. 2022 Jul;69(4):e356-e361. doi: 10.1111/tbed.14309. Epub 2021 Sep 12.

Abstract

Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc ) derived from normal prion protein (PrPC ), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPSc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPSc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPSc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.

Keywords: E200K; E211K; PMCA; PRNP; RT-QuIC; bovine spongiform encephalopathy; prion; prion disease; somatic mutation.

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases*
  • Encephalopathy, Bovine Spongiform* / diagnosis
  • Encephalopathy, Bovine Spongiform* / genetics
  • Mutation
  • Prion Diseases* / genetics
  • Prion Diseases* / veterinary
  • Prion Proteins / genetics
  • Prion Proteins / metabolism
  • Prions*

Substances

  • Prion Proteins
  • Prions