A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells

Fabio Hasler; Agathe Duda; Thomas M Kündig; Pål Johansen

doi:10.1016/j.xpro.2021.100824

A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells

STAR Protoc. 2021 Dec 17;2(4):100824. doi: 10.1016/j.xpro.2021.100824. Epub 2021 Aug 27.

Authors

Fabio Hasler¹, Agathe Duda², Thomas M Kündig^{1

2}, Pål Johansen^{1

2}

Affiliations

¹ Department of Dermatology, University of Zurich, 8091 Zurich, Switzerland.
² Department of Dermatology, University Hospital Zurich, 8091 Zurich, Switzerland.

Abstract

For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel.

Keywords: Cell culture; Cell-based Assays; Immunology; Microbiology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / blood*
Antibodies, Neutralizing / immunology
Antibodies, Viral / blood*
Antibodies, Viral / immunology
COVID-19 / blood
COVID-19 / immunology*
COVID-19 / therapy
Chlorocebus aethiops
Enzyme-Linked Immunosorbent Assay
Humans
Neutralization Tests / methods*
SARS-CoV-2 / immunology*
Vero Cells
Viral Plaque Assay / methods*

Substances

Antibodies, Neutralizing
Antibodies, Viral