CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene

Stem Cell Res. 2021 Oct:56:102519. doi: 10.1016/j.scr.2021.102519. Epub 2021 Aug 26.

Abstract

SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Epigenesis, Genetic
  • Histone Methyltransferases
  • Histones / genetics
  • Histones / metabolism
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Repressor Proteins / genetics

Substances

  • Histones
  • Repressor Proteins
  • SUV39H1 protein, human
  • Histone Methyltransferases
  • Methyltransferases