In vitro Assay to Assess Efficacy of Potential Antiviral Compounds against Enterovirus D68

Liang Sun; Leen Delang; Carmen Mirabelli; Johan Neyts

doi:10.21769/BioProtoc.2183

In vitro Assay to Assess Efficacy of Potential Antiviral Compounds against Enterovirus D68

Bio Protoc. 2017 Mar 20;7(6):e2183. doi: 10.21769/BioProtoc.2183.

Authors

Liang Sun¹, Leen Delang¹, Carmen Mirabelli¹, Johan Neyts¹

Affiliation

¹ KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Abstract

In 2014 enterovirus D68 (EV-D68) caused the largest outbreak in the United States since the discovery of the virus. Distinct from before, the 2014 infections were associated with more severe respiratory disease and occasional neurological complications. So far, there are no available vaccines or antivirals for the prophylaxis or treatment of EV-D68 infections. In order to evaluate the antiviral activity of potential inhibitors of EV-D68 replication, a cell-based cytopathic effect (CPE) reduction assay was developed ( Sun et al., 2015 ).

Keywords: Antiviral assay; CPE; Cell culture; Enterovirus D68; MTS/PMS solution.