Laser Doppler holography of the anterior segment for blood flow imaging, eye tracking, and transparency assessment

Léo Puyo; Clémentine David; Rana Saad; Sami Saad; Josselin Gautier; José Alain Sahel; Vincent Borderie; Michel Paques; Michael Atlan

doi:10.1364/BOE.425272

Laser Doppler holography of the anterior segment for blood flow imaging, eye tracking, and transparency assessment

Biomed Opt Express. 2021 Jun 28;12(7):4478-4495. doi: 10.1364/BOE.425272. eCollection 2021 Jul 1.

Authors

Léo Puyo^{1

2

3}, Clémentine David¹, Rana Saad^{1

2}, Sami Saad¹, Josselin Gautier^{1

2}, José Alain Sahel^{1

2

4

5}, Vincent Borderie¹, Michel Paques^{1

2

5}, Michael Atlan^{2

6}

Affiliations

¹ Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, INSERM-DHOS CIC 1423, 28 rue de Charenton, 75012 Paris, France.
² Paris Eye Imaging, France.
³ Institute of Biomedical Optics, University of Lübeck. Peter-Monnik-Weg 4, 23562 Lübeck, Germany.
⁴ Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
⁵ Institut de la Vision, Sorbonne Université, INSERM, CNRS, 17 Rue Moreau, 75012 Paris, France.
⁶ Institut Langevin, CNRS, PSL University, ESPCI Paris, 1 rue Jussieu, 75005 Paris, France.

Abstract

Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making changes to the instrument. Blood flow in the bulbar conjunctiva and episclera as well as in corneal neovascularization can be effectively imaged. We additionally demonstrate simultaneous holographic imaging of the anterior and posterior segments by simply adapting the numerical propagation distance to the plane of interest. We used this feature to track the movements of the retina and pupil with high temporal resolution. Finally, we show that the light backscattered by the retina can be used for retro-illumination of the anterior segment. Hence digital holography can reveal opacities caused by absorption or diffusion in the cornea and eye lens.