1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2

Biomol NMR Assign. 2021 Oct;15(2):467-474. doi: 10.1007/s12104-021-10047-2. Epub 2021 Aug 28.

Abstract

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1H, 13C and 15N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.

Keywords: 5'-UTR; COVID19-NMR; SARS-CoV-2; SL1; Solution NMR spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Nucleic Acid Conformation
  • RNA, Spliced Leader
  • SARS-CoV-2 / genetics*

Substances

  • 5' Untranslated Regions
  • RNA, Spliced Leader