Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
Keywords:
SARS-CoV-2; capping enzyme; coronavirus; methyltransferase; nsp10; nsp14; nsp16.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / analysis
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COVID-19 / virology*
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Humans
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Methyltransferases / analysis
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Methyltransferases / genetics
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Methyltransferases / metabolism*
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Protein Transport
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RNA Caps / genetics*
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RNA Caps / metabolism
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RNA, Viral / genetics*
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RNA, Viral / metabolism
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SARS-CoV-2 / chemistry
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SARS-CoV-2 / enzymology*
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SARS-CoV-2 / genetics
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Viral Nonstructural Proteins / analysis
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism*
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Viral Regulatory and Accessory Proteins / analysis
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Viral Regulatory and Accessory Proteins / genetics
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Viral Regulatory and Accessory Proteins / metabolism*
Substances
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Antibodies, Monoclonal
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NSP10 protein, SARS-CoV-2
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NSP16 protein, SARS-CoV-2
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RNA Caps
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RNA, Viral
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Viral Nonstructural Proteins
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Viral Regulatory and Accessory Proteins
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Methyltransferases