The wide application of graphene oxide (GO) increases its release into environment with less known on environmental effects. This work investigated 120-day interaction between GO (500 and 5000 μg/L) and constructed wetlands (CWs) planted with Iris pseudacorus. CWs showed the effective retention for GO via mature biofilm but less biodegradation. GO significantly induced enzyme activities (urease, neutral phosphatase, and catalase), which was attributed to increases in ecological association and enzyme abundance. GO decreased microbial biomass on day 30, but it had no impacts on day 120. The microbial community showed gradual self-adaption with time due to protection of antioxidant defense system (L-ascorbate oxidase, superoxide reductase, and glutathione related enzyme). The antioxidant enzymes (superoxide dismutase and peroxidase) and lipid peroxidation of Iris pseudacorus were increased by GO, accompanied by reduction on chlorophyll biosynthesis. Overall, the separate effects of GO on micro-regions and individual bodies in CWs were obvious, but it was acceptable that variations in pollutant removal were not evident due to synergetic role of plant-substrate-microbe. Organic matter and phosphorus removals reached to above 93%, and ammonia and total nitrogen removals in GO groups were reduced by 7-8% and 9-13%, respectively.
Keywords: Antioxidant defense system; Constructed wetlands; Enzyme activities; Graphene oxide; Nutrient removal.
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