Genome-wide analysis of BBX gene family in Tartary buckwheat (Fagopyrum tataricum)

Jiali Zhao; Hongyou Li; Juan Huang; Taoxiong Shi; Ziye Meng; Qingfu Chen; Jiao Deng

doi:10.7717/peerj.11939

Genome-wide analysis of BBX gene family in Tartary buckwheat (Fagopyrum tataricum)

PeerJ. 2021 Aug 11:9:e11939. doi: 10.7717/peerj.11939. eCollection 2021.

Authors

Jiali Zhao¹, Hongyou Li¹, Juan Huang¹, Taoxiong Shi¹, Ziye Meng¹, Qingfu Chen¹, Jiao Deng¹

Affiliation

¹ School of Life Sciences, Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, China.

Abstract

BBX (B-box), a zinc finger transcription factor with one or two B-box domains, plays an important role in plant photomorphogenesis, growth, and development as well as response to environmental changes. In this study, 28 Tartary buckwheat BBX (FtBBX) genes were identified and screened using a comparison program. Their physicochemical properties, gene structures, conserved motifs, distribution in chromosomal, and phylogeny of the coding proteins, as well as their expression patterns, were analyzed. In addition, multiple collinearity analysis in three monocots and three dicot species illustrated that the BBX proteins identified from monocots clustered separately from those of dicots. Moreover, the expression of 11 candidate BBX genes with probable involvement in the regulation of anthocyanin biosynthesis was analyzed in the sprouts of Tartary buckwheat during light treatment. The results of gene structure analysis showed that all the 28 BBX genes contained B-box domain, three genes lacked introns, and these genes were unevenly distributed on the other seven chromosomes except for chromosome 6. The 28 proteins contained 10 conserved motifs and could be divided into five subfamilies. BBX genes of Tartary buckwheat showed varying expression under different conditions demonstrating that FtBBXs might play important roles in Tartary buckwheat growth and development. This study lays a foundation for further understanding of Tartary buckwheat BBX genes and their functions in growth and development as well as regulation of pigmentation in Tartary buckwheat.

Keywords: Anthocyanin biosynthesis; Regulation.

Grants and funding

This work was supported by the National Natural Science Foundation of China-Project of Karst Science Research Center of Guizhou Provincial People’s Government (U1812401), the Science and Technology Foundation of Guizhou Province (QianKeHeJiChu [2020]1Y095), the Guizhou Provincial Department of Education Youth Science and Technology Talent Growth Project (Qianjiaohe KY Zi (2018)128), the National Natural Science Foundation of China (31701494, 31760419), the Initial Fund for Doctor Research in Guizhou Normal University (11904/0516026), and the Earmarked Fund for Construction of the Key Laboratory for Conservation and Innovation of Buckwheat Germplasm in Guizhou (QianJiaoHe KY Zi (2017)002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.