Comparative Genome Analysis Provides Molecular Evidence for Reclassification of the Photosynthetic Bacterium Rhodobacter sphaeroides EBL0706 as a Strain of Luteovulum azotoformans

Haoyu Wang; Xiaoling Sha; Rui Li; Yijing Li; Himel Nahreen Khaleque; Yuxiu Zhang; Tsing Bohu; Zhihui Bai; Xuliang Zhuang

doi:10.3390/microorganisms9081754

Comparative Genome Analysis Provides Molecular Evidence for Reclassification of the Photosynthetic Bacterium Rhodobacter sphaeroides EBL0706 as a Strain of Luteovulum azotoformans

Microorganisms. 2021 Aug 17;9(8):1754. doi: 10.3390/microorganisms9081754.

Authors

Haoyu Wang^{1

2}, Xiaoling Sha^{2

3}, Rui Li^{2

3

4}, Yijing Li^{2

3}, Himel Nahreen Khaleque⁵, Yuxiu Zhang¹, Tsing Bohu^{6

7}, Zhihui Bai^{2

3}, Xuliang Zhuang^{2

3}

Affiliations

¹ School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China.
² Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
³ University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Beijing Huachen Jiguang Technology, Co. Ltd., Beijing 101407, China.
⁵ CSIRO Land and Water, Private Bag No. 5, Wembley, WA 6913, Australia.
⁶ State Key Laboratory of Lunar and Planetary Sciences, Macau University of Science and Technology, Taipa 999078, Macau.
⁷ CSIRO Mineral Resources, Australian Resources and Research Centre, Kensington, WA 6151, Australia.

Abstract

In this study, we conducted a genome-wide comparative analysis of a former Rhodobacter sphaeroides strain EBL0706, which is now recorded as Luteovulum sphaeroides EBL0706. The genome of EBL0706 was compared with that of Luteovulum azotoformans ATCC 17025, Luteovulum azotoformans KA25, and Luteovulum sphaeroides 2.4.1. The average nucleotide identity (ANI), tetra nucleotide signatures (Tetra), digital DNA-DNA hybridization (dDDH) values, comparative genome, and phylogenetic analysis proposed that EBL0706 is a strain of Luteovulum azotoformans. Functional annotations identified a total of 4034 protein-coding genes in the genome of EBL0706, including a complete photosynthetic gene cluster. This study provides genomic molecular verification for the strain EBL0706 to be reclassified to Luteovulum azotoformans.

Keywords: Luteovulum azotoformans; Rhodobacter sphaeroides; genome comparison; phylogenetic; reclassification.

Abstract

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