Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China

Wang Xiaoting; Ning Chengcheng; Ji Chunhui; Li Yan; Li Jing; Meng Qingling; Qiao Jun; Wang Lixia; Cai Kuojun; Zhang Jinsheng; Zhang Zaichao; Yu Weiwei; Peng Yelong; Cai Xuepeng

doi:10.1016/j.jgar.2021.08.003

Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China

J Glob Antimicrob Resist. 2021 Dec:27:41-45. doi: 10.1016/j.jgar.2021.08.003. Epub 2021 Aug 23.

Authors

Wang Xiaoting¹, Ning Chengcheng², Ji Chunhui², Li Yan², Li Jing², Meng Qingling², Qiao Jun³, Wang Lixia², Cai Kuojun⁴, Zhang Jinsheng⁵, Zhang Zaichao⁶, Yu Weiwei⁷, Peng Yelong⁸, Cai Xuepeng⁹

Affiliations

¹ College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, China; Department of Animal Laboratory, Chifeng Municipal Hospital, Chifeng, Inner Mongolia, 024000, China.
² College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, China.
³ College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, China. Electronic address: qj710625@shzu.edu.cn.
⁴ Center for Animal Disease Prevention and Control, Urumqi, Xinjiang, 830000, China.
⁵ Center for Animal Disease Prevention and Control, Tacheng, Xinjiang, 834700, China.
⁶ Center for Animal Disease Prevention and Control, Changji, Xinjiang, 831500, China.
⁷ Center for Animal Disease Prevention and Control, Korla, Xinjiang, 841000, China.
⁸ Center for Animal Disease Prevention and Control, Aksu, Xinjiang, 8430000, China.
⁹ State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China.

PMID: 34438107
DOI: 10.1016/j.jgar.2021.08.003

Abstract

Objectives: Clostridium perfringens (C. perfringens) can cause intestinal diseases in livestock and humans, which seriously threatens the healthy development of animal husbandry and human food safety. Here, the characteristics of antimicrobial resistance and molecular typing of ruminant-borne strains of C. perfringens in Xinjiang, China were explored and profiled.

Methods: A total of 307 clinical feces collected from ruminants (cattle and sheep) with diarrheal symptoms were screened for C. perfringens. The recovered isolates were characterized in respect to their antimicrobial resistance pattern and molecular typing.

Results: A total of 109 isolates of C. perfringens were isolated from 307 clinical feces of ruminants, most of which displayed the multidrug resistance (MDR) phenotype. Demonstration of the quinolone-resistance gene was the highest among the isolates (70.6%). The multiplex PCR typing based on toxin genes showed that type A and type D strains made up 82.6% (90/109) and 17.4% (19/109), among which, the isolates carrying β2 gene occupied 43.3% (39/90) of type A strains and 31.6% (6/19) of type D strains. These isolates were divided into 6 genotypes (I-VI) by enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) method. A total of 33 ST types (ST1-ST33) were identified by multilocus sequence typing (MLST) method.

Conclusion: C. perfringens isolates with multidrug resistance (MDR) were frequent and circulating in ruminants. Among them, type A-Ⅰ-ST19 was the dominant genotype of C. perfringens, displaying obvious genetic diversity. This study provided important epidemiological data for the risk assessment of food safety associated with ruminant-borne C. perfringens in Xinjiang, China.

Keywords: Antimicrobial resistance profiles; Clostridium perfringens; Molecular typing; Ruminant-borne isolates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Cattle
China / epidemiology
Clostridium Infections* / epidemiology
Clostridium Infections* / veterinary
Clostridium perfringens* / genetics
Drug Resistance, Bacterial
Multilocus Sequence Typing
Ruminants
Sheep

Substances

Anti-Bacterial Agents